An efficient DDAB-mediated transfection of Drosophila S2 cells.
نویسنده
چکیده
I have developed an efficient method for transfecting Drosophila S2 cells using DDAB, a cationic liposome reagent. The optimized DDAB method resulted in a 10 times or greater increase in transfection efficiency compared with the conventional calcium phosphate method which has been essentially the only way for transfecting S2 cells.
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Drosophila melanogaster Schneider line 2 (S2) cells are useful for in vitro analysis of mammalian and non-mammalian genes or proteins. Despite their multiple uses and the recent advancements in transfection technologies, S2 cells are still typically transfected using the relatively inefficient calcium phosphate method. We report the ability to greatly increase transfection efficiency with S2 ce...
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عنوان ژورنال:
- Nucleic acids research
دوره 24 21 شماره
صفحات -
تاریخ انتشار 1996